2014 - Video-animace-časosběr: "Useless" S. pombe imaging
The intention was to compare the cell cycle duration of two S. pombe strains with different genetic background. To distinguish between the strains we used the cells with fluorescently labeled nucleolar protein gar2 using GFP and mCherry taggs. Unfortunately, the insufficient humidification of the heating chambre caused the solid medium agar slice shrinking which resulted in the medium concentration changes and focus-area movement. 10 hours of microscopy showed no results.